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Analysis of electrical properties of cardiac cells
Švecová, Olga ; Čmiel, Vratislav (referee) ; Šimurda, Jiří (advisor)
The diploma thesis is focused on the analysis of the electrical properties of the heart cells membranes. The main goal of this work is to answer to what extent it is possible to infer from experimental data on the detailed mechanism of interaction of substances with channels. The work also presents a detailed description of methods of measurement of membrane voltage and current, which are used in the experimental measurements. There are also discussed the individual channels of the cell membrane and ion currents, which arise as a response to the rectangular pulse voltage. There is also described the effect of various substances on the properties of ion currents flowing through the cell membrane.
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Detection of cardiac cells in microscopic image
Musikhina, Ksenia ; Hrubeš, Jan (referee) ; Rychtárik, Milan (advisor)
This work is devoted to problem of detection of cardiac cells in microscopic picture. All possible means of preprocessing and segmentation were considered with the aim to choose the most suitable method for further classification. Different methods of classification were be testing: method of objects attributes and classifier based on neural network. As a result was obtained the number of living and dead cardiac cells and percentage of them. The electivity of classification methods was calculated by sensitivity and specificity. The user’s interface was created for improvement of clearness classification in MATLAB environment.
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Modeling of calcium dynamics in excitable and non-excitable cells
Tkáč, Jan ; Čmiel, Vratislav (referee) ; Rychtárik, Milan (advisor)
This bachelor thesis deals with electrophysiologic phenomenons on molecular and cellular level. The aim is to provide basic information for better understanding of simple cellular models. This work also discusses principles of calcium dynamic modelling in cells. In Matlab environment the Lou-Rudy model from 1991, Lou-Rudy model from 1994 and the phantom burster model for pancreatic ß-cells are implemented. Using the GUI interface a clear program for simulation electrophysiologic phenomenons is created.
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Compartmentalization of the beta-adrenergic signaling system in cardiac cells: the effect of hypoxia
Karlovská, Ivana ; Novotný, Jiří (advisor) ; Nováková, Olga (referee)
The aim of this thesis was to study the changes that occur in cell line H9c2 after exposure to an oxygen level reduced to 2 % for 24 hours. We monitored changes in compartmentation of chosen members of β-adrenergic signaling system. We found an increase in expression of β1AR and β2AR. Only β2AR showed change in compartmentation after hypoxia, as they relocate from membrane rafts to non-rafts fractions of membrane. AC also showed an increase of expression and was located in membrane rafts. The next aim of this work was to monitore apoptotic markers to determine whether there are activated pro-apoptotic or anti-apoptotic signals under chosen conditions of hypoxia. There was an increase in expression of both pro-apoptotic protein Bax and anti-apoptotic protein Bcl-2. We compare ratios of Bcl-2 to Bax and we found that there is a bigger increase in protein Bax expression. Another apoptotic marker, caspase 3, was tested and we also found that there was an increase in expression of caspase 3 in cells after hypoxia. Furthermore, we studied possible activation of kinase signaling pathways that may contribute to protective effects of hypoxia. Expression of Akt and ERK kinases was increased after hypoxia, but we did not confirm activation by phosphorylation of these kinases. Levels of phosphorylated Akt...
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Modeling of calcium dynamics in excitable and non-excitable cells
Tkáč, Jan ; Čmiel, Vratislav (referee) ; Rychtárik, Milan (advisor)
This bachelor thesis deals with electrophysiologic phenomenons on molecular and cellular level. The aim is to provide basic information for better understanding of simple cellular models. This work also discusses principles of calcium dynamic modelling in cells. In Matlab environment the Lou-Rudy model from 1991, Lou-Rudy model from 1994 and the phantom burster model for pancreatic ß-cells are implemented. Using the GUI interface a clear program for simulation electrophysiologic phenomenons is created.
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Analysis of electrical properties of cardiac cells
Švecová, Olga ; Čmiel, Vratislav (referee) ; Šimurda, Jiří (advisor)
The diploma thesis is focused on the analysis of the electrical properties of the heart cells membranes. The main goal of this work is to answer to what extent it is possible to infer from experimental data on the detailed mechanism of interaction of substances with channels. The work also presents a detailed description of methods of measurement of membrane voltage and current, which are used in the experimental measurements. There are also discussed the individual channels of the cell membrane and ion currents, which arise as a response to the rectangular pulse voltage. There is also described the effect of various substances on the properties of ion currents flowing through the cell membrane.
|
|
|
Detection of cardiac cells in microscopic image
Musikhina, Ksenia ; Hrubeš, Jan (referee) ; Rychtárik, Milan (advisor)
This work is devoted to problem of detection of cardiac cells in microscopic picture. All possible means of preprocessing and segmentation were considered with the aim to choose the most suitable method for further classification. Different methods of classification were be testing: method of objects attributes and classifier based on neural network. As a result was obtained the number of living and dead cardiac cells and percentage of them. The electivity of classification methods was calculated by sensitivity and specificity. The user’s interface was created for improvement of clearness classification in MATLAB environment.
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